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pkh26 red fluorescent dye  (MedChemExpress)
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pkh26 fluorescent dye  (Yeasen Biotechnology)
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Baricitinib mitigates BLM-induced HSF fibrotic activation via HUVEC exosome secretion. A Nanoparticle Tracking Analysis (NTA) of the size distribution and concentration of exosomes derived from HUVECs. B Transmission Electron Microscopy (TEM) images showing the morphology of exosomes derived from HUVECs. C Representative Western blot images showing protein expression of exosomal biomarkers (CD9, CD63, CD81). D Schematic diagram of the experimental design: Exosomes (EXO) were collected from Baricitinib-treated HUVECs and applied to BLM-induced HSFs. E CCK-8 assay showing the proliferation of BLM-induced HSFs treated with EXO from differently treated HUVECs. F Fluorescent images demonstrating the internalization of <t>PKH26-labeled</t> EXO (red) by BLM-induced HSFs (nuclei counterstained with DAPI, blue). G Representative fluorescent images of intracellular actin filaments (stained with Alexa Fluor® 488-conjugated phalloidin) in HSFs treated with the indicated EXO. Scale bar = 50 μm. H Representative fluorescent images of α-SMA expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. I Representative fluorescent images of Collagen III expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. J RT-qPCR analysis of mRNA expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. K Representative Western blot images (left) and densitometric analysis (right) of protein expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. Data represent mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Pkh26 Fluorescent Dye, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 fluorescent dye solution  (Merck & Co)
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Baricitinib mitigates BLM-induced HSF fibrotic activation via HUVEC exosome secretion. A Nanoparticle Tracking Analysis (NTA) of the size distribution and concentration of exosomes derived from HUVECs. B Transmission Electron Microscopy (TEM) images showing the morphology of exosomes derived from HUVECs. C Representative Western blot images showing protein expression of exosomal biomarkers (CD9, CD63, CD81). D Schematic diagram of the experimental design: Exosomes (EXO) were collected from Baricitinib-treated HUVECs and applied to BLM-induced HSFs. E CCK-8 assay showing the proliferation of BLM-induced HSFs treated with EXO from differently treated HUVECs. F Fluorescent images demonstrating the internalization of <t>PKH26-labeled</t> EXO (red) by BLM-induced HSFs (nuclei counterstained with DAPI, blue). G Representative fluorescent images of intracellular actin filaments (stained with Alexa Fluor® 488-conjugated phalloidin) in HSFs treated with the indicated EXO. Scale bar = 50 μm. H Representative fluorescent images of α-SMA expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. I Representative fluorescent images of Collagen III expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. J RT-qPCR analysis of mRNA expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. K Representative Western blot images (left) and densitometric analysis (right) of protein expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. Data represent mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Pkh26 Fluorescent Dye Solution, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 fluorescent dye solution - by Bioz Stars, 2026-10
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red fluorescence dye pkh26  (Millipore)
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Baricitinib mitigates BLM-induced HSF fibrotic activation via HUVEC exosome secretion. A Nanoparticle Tracking Analysis (NTA) of the size distribution and concentration of exosomes derived from HUVECs. B Transmission Electron Microscopy (TEM) images showing the morphology of exosomes derived from HUVECs. C Representative Western blot images showing protein expression of exosomal biomarkers (CD9, CD63, CD81). D Schematic diagram of the experimental design: Exosomes (EXO) were collected from Baricitinib-treated HUVECs and applied to BLM-induced HSFs. E CCK-8 assay showing the proliferation of BLM-induced HSFs treated with EXO from differently treated HUVECs. F Fluorescent images demonstrating the internalization of <t>PKH26-labeled</t> EXO (red) by BLM-induced HSFs (nuclei counterstained with DAPI, blue). G Representative fluorescent images of intracellular actin filaments (stained with Alexa Fluor® 488-conjugated phalloidin) in HSFs treated with the indicated EXO. Scale bar = 50 μm. H Representative fluorescent images of α-SMA expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. I Representative fluorescent images of Collagen III expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. J RT-qPCR analysis of mRNA expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. K Representative Western blot images (left) and densitometric analysis (right) of protein expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. Data represent mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Red Fluorescence Dye Pkh26, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Baricitinib mitigates BLM-induced HSF fibrotic activation via HUVEC exosome secretion. A Nanoparticle Tracking Analysis (NTA) of the size distribution and concentration of exosomes derived from HUVECs. B Transmission Electron Microscopy (TEM) images showing the morphology of exosomes derived from HUVECs. C Representative Western blot images showing protein expression of exosomal biomarkers (CD9, CD63, CD81). D Schematic diagram of the experimental design: Exosomes (EXO) were collected from Baricitinib-treated HUVECs and applied to BLM-induced HSFs. E CCK-8 assay showing the proliferation of BLM-induced HSFs treated with EXO from differently treated HUVECs. F Fluorescent images demonstrating the internalization of PKH26-labeled EXO (red) by BLM-induced HSFs (nuclei counterstained with DAPI, blue). G Representative fluorescent images of intracellular actin filaments (stained with Alexa Fluor® 488-conjugated phalloidin) in HSFs treated with the indicated EXO. Scale bar = 50 μm. H Representative fluorescent images of α-SMA expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. I Representative fluorescent images of Collagen III expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. J RT-qPCR analysis of mRNA expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. K Representative Western blot images (left) and densitometric analysis (right) of protein expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. Data represent mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Journal: Arthritis Research & Therapy

Article Title: Baricitinib ameliorates skin fibrosis via direct fibroblast suppression and endothelial exosome-mediated paracrine signaling

doi: 10.1186/s13075-025-03710-9

Figure Lengend Snippet: Baricitinib mitigates BLM-induced HSF fibrotic activation via HUVEC exosome secretion. A Nanoparticle Tracking Analysis (NTA) of the size distribution and concentration of exosomes derived from HUVECs. B Transmission Electron Microscopy (TEM) images showing the morphology of exosomes derived from HUVECs. C Representative Western blot images showing protein expression of exosomal biomarkers (CD9, CD63, CD81). D Schematic diagram of the experimental design: Exosomes (EXO) were collected from Baricitinib-treated HUVECs and applied to BLM-induced HSFs. E CCK-8 assay showing the proliferation of BLM-induced HSFs treated with EXO from differently treated HUVECs. F Fluorescent images demonstrating the internalization of PKH26-labeled EXO (red) by BLM-induced HSFs (nuclei counterstained with DAPI, blue). G Representative fluorescent images of intracellular actin filaments (stained with Alexa Fluor® 488-conjugated phalloidin) in HSFs treated with the indicated EXO. Scale bar = 50 μm. H Representative fluorescent images of α-SMA expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. I Representative fluorescent images of Collagen III expression in BLM-induced HSFs treated with the indicated EXO. Scale bar = 50 μm. J RT-qPCR analysis of mRNA expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. K Representative Western blot images (left) and densitometric analysis (right) of protein expression levels of fibrosis-related markers (α-SMA, Collagen I, Collagen III) in HSFs treated with the indicated EXO. Data represent mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Purified exosomes were labeled with 2 μM PKH26 fluorescent dye (#40780ES20, YEASEN) for 5 min at RT, with staining quenched by adding 1% BSA.

Techniques: Activation Assay, Concentration Assay, Derivative Assay, Transmission Assay, Electron Microscopy, Western Blot, Expressing, CCK-8 Assay, Labeling, Staining, Quantitative RT-PCR